Interestingly, to our knowledge, this is the first report of Leishmania detection in Hydrochaeris hydrochaeris (capybara) in Colombia. braziliensis was observed in samples from wild mammals. Alpha and beta diversity indices were significant, mainly for dogs there was an interesting index of coinfection events in the analyzed samples: different Leishmania species and the simultaneous presence of T. Comparison of the identified species by the two sequencing technologies used resulted in 97% concordance. One hundred and thirteen samples were positive for PCR by Hsp70 fragment these corresponded to 22.1% Leishmania spp., 18.6% L. Data obtained were used to perform diversity analyses. Twenty-seven samples were sent for amplicon-based NGS using the same gene fragment. A total of 174 DNA samples (18 humans, 83 dogs, and 73 wild mammals) were analyzed by conventional PCR using a fragment of the heat shock protein 70 ( Hsp70) gene and Sanger sequenced the positive samples. We used Sanger and amplicon-based next-generation sequencing (NGS) in samples from different mammals to identify trypanosomatid infections in several departments in Colombia.

Diseases associated with these pathogens manifest mainly in poor and vulnerable populations, where social, environmental, and biological factors modulate the case incidence and geographical distribution. Trypanosomatids are among the most critical parasites for public health due to their impact on human, animal, and plant health.

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